Lyss

常见例句

Methods The gene encoding wide-type p53 was isolated,using RT-PCR from A549 cell line and cloned into pTATHA and pET32a prokaryotic expression vectors. Recombinant plasmids were E. coli BL21(DE3)LysS,then the transformed cells were induced with IPTG.
方法:利用RT-PCR方法从A549细胞系中分离野生型p53基因,将该基因分别克隆入pTATHA和pET32 a原核表达载体,在大肠杆菌BL21(DE3)LysS内诱导表达并进行纯化。
The GST-ORF5 fusion protein has been expressed in Escherichia coli BL21 (DE3) LysS cells and anti-GST-ORF5 antiserum has been prepared. Characterization of ORF5product needs to be carried out further.
构建了ORF5与pGEX-4T-2的GST融合表达载体,在大肠杆菌BL21中得到了表达,并制备了兔多克隆抗体。
coli BL21(DE3)LysS and expressed with the induction of IPTG. The expression of the fusion proteins was analyzed by SDS-PAGE and Western blot.
在大肠杆菌BL21(DE3)LysS内，以IPTG诱导融合蛋白表达，表达产物用SDS-PAGE及Western blot分析鉴定。