over-lap pcr

The two tandem fragment of Aβ obtaining by overlap PCR technique was cloned into plant expression vector pBIDST(containing double 35S promoter and TEV enhancer).
本试验利用重叠PCR技术获得2Aβ串联基因片段,并将其克隆到植物表达载体pBIDST(含双35S启动子和TEV增强子)中。